PODXL anticorps (Extracellular Domain, Internal Region)

N° du produit ABIN3025409

Cet anticorps Souris Monoclonal détecte spécifiquement PODXL dans FACS, IF et IHC (p). Il présente une réactivité envers Humain, Rat et Lapin.

Aperçu rapide pour PODXL anticorps (Extracellular Domain, Internal Region) (ABIN3025409)

Antigène: PODXL (Podocalyxin-Like (PODXL))

Reactivité: Humain, Rat, Lapin

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp PODXL est non-conjugé

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 2A4

Détail du produit anti-PODXL anticorps

Épitope: Extracellular Domain, Internal Region

Purification: PEG precipitation

Immunogène: A recombinant protein fragment containing the intracellular, transmembrane, and part of the extracellular domain of human PODXL was used as the immunogen for the Podocalyxin antibody.

Isotype: IgM

Information d'application

Indications d'application: Optimal dilution of the Podocalyxin antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the Podocalyxin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur PODXL

Antigène: PODXL (Podocalyxin-Like (PODXL))

Autre désignation: Podocalyxin

Sujet: Podocalyxin/PODXL is a member of the CD34 transmembrane sialomucin family. It is over-expressed on the podocyte foot projections and plays essential roles in kidney development and homeostasis, blood filtration and urine formation. It is also expressed on vascular endothelia, hematopoietic progenitors and a subset of neurons. Overexpression of podocalyxin may be linked to more aggressive tumor behavior. Podocalyxin antibody can identify podocytes in the urine (podocyturia) that may indicate glomerular disease, pre-eclampsia, and other kidney pathology.

Pathways: Tube Formation